Acetylhydrolase-IN-1 is primarily utilized in biochemical research to study the role of acetylhydrolase enzymes in various biological processes. It serves as an inhibitor, helping researchers understand the enzyme's function in lipid metabolism and its involvement in inflammatory pathways. This compound is particularly valuable in exploring conditions like atherosclerosis, where acetylhydrolase activity is significant. Additionally, it aids in the development of therapeutic strategies targeting enzyme-related disorders, providing insights into potential drug design and efficacy. Its application extends to cellular and molecular studies, where it helps elucidate mechanisms underlying enzyme regulation and its impact on cellular signaling.