DUBs inhibitor 3 is primarily utilized in biochemical and pharmacological research to study the role of deubiquitinating enzymes (DUBs) in cellular processes. It is commonly applied in experiments to investigate protein degradation pathways, ubiquitin signaling, and the regulation of protein stability. Researchers use this inhibitor to explore its effects on cancer cell lines, as DUBs are often implicated in tumor progression and resistance to therapy. Additionally, it serves as a tool compound in drug discovery efforts aimed at developing targeted therapies for diseases where DUB activity is dysregulated, such as neurodegenerative disorders and autoimmune conditions. Its application also extends to understanding the molecular mechanisms underlying immune response and inflammation.