Arphamenine B hemisulfate is primarily utilized in biochemical research as a potent inhibitor of aminopeptidases, enzymes that play a critical role in protein metabolism. Its application is significant in studying the mechanisms of enzyme activity and regulation, particularly in the context of cellular processes and disease pathways. Researchers employ it to investigate the role of aminopeptidases in various physiological and pathological conditions, such as cancer, immune response, and neurodegenerative diseases. Additionally, it serves as a valuable tool in drug discovery, helping to identify and develop therapeutic agents that target aminopeptidase-related pathways. Its use in laboratory settings aids in understanding protein degradation and peptide processing, contributing to advancements in biomedical science.