Blebbistatin is widely used in research as a selective inhibitor of myosin II, making it valuable for studying cell motility, cytokinesis, and muscle contraction. It is particularly useful in experiments investigating the role of non-muscle myosin II in processes like cell migration, division, and tissue organization. In developmental biology, it helps explore the mechanisms of morphogenesis and organ formation. Additionally, it is employed in biophysics to study the mechanics of actomyosin interactions and their role in cellular force generation. Blebbistatin is also used in imaging studies to reduce phototoxicity and improve the quality of live-cell microscopy. Its ability to reversibly inhibit myosin II activity makes it a versatile tool in both in vitro and in vivo experimental models.