ACAT-IN-1 cis isomer is primarily utilized in biochemical research to study the inhibition of acyl-CoA:cholesterol acyltransferase (ACAT) enzymes. These enzymes play a crucial role in cholesterol esterification, a process essential for cellular cholesterol homeostasis. By inhibiting ACAT, this compound helps researchers investigate the mechanisms of cholesterol metabolism and its impact on diseases such as atherosclerosis and hypercholesterolemia. It is also used in drug discovery programs aimed at developing therapeutic agents for cardiovascular and metabolic disorders. Additionally, ACAT-IN-1 cis isomer serves as a valuable tool in understanding lipid storage and transport within cells, contributing to advancements in lipid biology and related pathologies.