MRT68921 hydrochloride is primarily utilized in biochemical research to study autophagy, a critical cellular process involved in the degradation and recycling of cellular components. It acts as a potent inhibitor of ULK1, a key kinase that regulates the initiation of autophagy. Researchers employ this compound to investigate the mechanisms of autophagy and its role in various physiological and pathological conditions, such as cancer, neurodegenerative diseases, and infections. By inhibiting ULK1, it helps elucidate the pathways and potential therapeutic targets for diseases where autophagy is dysregulated. Additionally, it is used in drug discovery and development to screen and validate compounds targeting autophagy-related processes.