Antipain is primarily used in biochemical research as a protease inhibitor, effectively blocking the activity of certain enzymes that break down proteins. It is particularly valuable in studies involving the purification and characterization of proteases, where it helps prevent unwanted degradation of target proteins. Additionally, antipain is utilized in cell culture experiments to inhibit proteolytic enzymes, ensuring the stability and integrity of cellular proteins during analysis. Its application extends to investigating cellular processes like apoptosis and signal transduction, where protease activity plays a critical role. Researchers also employ antipain to study the mechanisms of enzyme inhibition and to develop potential therapeutic strategies targeting protease-related diseases.