BPTU is primarily utilized in biochemical research as a selective inhibitor of protein kinases. It is particularly effective in studying the signaling pathways and cellular processes that are regulated by these enzymes. Researchers employ BPTU to investigate the role of specific kinases in disease mechanisms, such as cancer and inflammatory disorders. By inhibiting kinase activity, BPTU helps in understanding the potential therapeutic targets for developing new drugs. Additionally, it is used in high-throughput screening assays to identify novel kinase inhibitors, aiding in drug discovery efforts. Its application extends to studying cellular responses and signal transduction pathways, providing insights into complex biological processes.