LY 294002, Hydrochloride Salt is widely used in biochemical and pharmacological research due to its role as a potent inhibitor of phosphoinositide 3-kinase (PI3K). It is particularly valuable in studying the PI3K/AKT signaling pathway, which is crucial for understanding cellular processes such as growth, proliferation, and survival. Researchers utilize this compound to investigate its effects on cancer cell lines, as PI3K signaling is often dysregulated in various cancers. Additionally, it is employed in studies exploring mechanisms of insulin resistance and metabolic disorders, given the pathway's involvement in glucose metabolism. Its application extends to neurobiology, where it helps in examining neuronal survival and apoptosis. Overall, LY 294002 serves as a critical tool in elucidating the molecular mechanisms underlying numerous diseases and biological functions.