1-Ethylguanidine hemisulfate is primarily used in biochemical research as a tool to study enzyme activity and protein interactions. It serves as a guanidine derivative, often employed to investigate the effects of guanidine compounds on biological systems, particularly in the study of protein denaturation and refolding processes. Its application extends to the examination of enzyme kinetics, where it helps in understanding the mechanisms of enzyme inhibition or activation. Additionally, it is utilized in the development of pharmaceuticals, particularly in the synthesis of compounds that target specific biochemical pathways. Its role in research contributes to advancements in molecular biology and drug discovery.