Porcupine-IN-1 is primarily used in research focused on inhibiting the Porcupine enzyme, which plays a crucial role in the Wnt signaling pathway. This pathway is involved in various cellular processes, including cell proliferation, differentiation, and tissue regeneration. By inhibiting Porcupine, this compound helps in studying the effects of Wnt signaling suppression, which is particularly relevant in cancer research. It is used to explore potential therapeutic strategies for cancers that are driven by aberrant Wnt signaling, such as colorectal and breast cancers. Additionally, it aids in understanding developmental biology and tissue homeostasis, providing insights into diseases linked to Wnt pathway dysregulation. Its application extends to preclinical studies aimed at developing targeted therapies for Wnt-dependent malignancies.