Used in biochemical research as a probe to study enzyme mechanisms, particularly in nicotinamide adenine dinucleotide (NAD)-dependent systems. Its sulfonated structure enhances water solubility, making it suitable for assays in aqueous environments. Acts as a modified cofactor analog to inhibit or track the activity of dehydrogenases and sirtuins. Also employed in the development of diagnostic tools for detecting enzymatic activity in cell lysates and clinical samples.