Sirt2-IN-9 is a potent and selective inhibitor of SIRT2, a member of the sirtuin family of NAD+-dependent deacetylases. It is widely used in biochemical and cellular research to study the role of SIRT2 in various biological processes. One of its primary applications is in cancer research, where inhibition of SIRT2 has been shown to affect cell cycle progression, promote tubulin acetylation, and induce apoptosis in certain tumor cell lines. It is also employed in neurodegenerative disease studies, as SIRT2 inhibition may offer protective effects in models of Parkinson’s and Huntington’s diseases by modulating protein aggregation and improving neuronal survival. Additionally, Sirt2-IN-9 serves as a valuable tool compound in epigenetic research, helping to dissect the involvement of SIRT2 in gene expression regulation and chromatin dynamics. Its selectivity makes it suitable for target validation and pathway analysis in both in vitro and cell-based assays.