Sirtinol is primarily used as a research tool in biological and biochemical studies due to its ability to inhibit sirtuin enzymes, particularly SIRT1 and SIRT2. These enzymes are involved in critical cellular processes such as aging, metabolism, DNA repair, and apoptosis. By inhibiting sirtuins, sirtinol helps scientists investigate the roles these enzymes play in various pathways and disease models. It has been applied in studies related to cancer, where sirtuin inhibition can lead to increased acetylation of key proteins like p53, promoting cell cycle arrest or apoptosis in tumor cells. Researchers also use sirtinol in aging and neurodegenerative disease research to explore how modulation of sirtuin activity affects neuronal survival and function. Additionally, sirtinol has been employed in metabolic studies to examine insulin sensitivity and glucose homeostasis, as sirtuins regulate metabolic pathways. Its use remains confined to laboratory settings, mainly in cell cultures and animal models, aiding the development of potential therapeutic strategies targeting sirtuin-related mechanisms.