Nexinhib20 is primarily used as a selective inhibitor of NEDD4 family of E3 ubiquitin ligases, particularly targeting NEDD4 and NEDD4L. It is widely applied in cellular and molecular research to study ubiquitin-mediated signaling pathways. By blocking the activity of these ligases, Nexinhib20 helps regulate the stability and trafficking of various membrane proteins, including ion channels, transporters, and receptors.

One key application is in cancer research, where Nexinhib20 has shown potential in suppressing tumor cell proliferation and migration, especially in lung and breast cancer models. It modulates key oncogenic pathways by preventing the degradation of tumor suppressor proteins that are normally targeted by NEDD4 ligases.

In neuroscience, Nexinhib20 is used to investigate the role of NEDD4 proteins in neuronal development, synaptic plasticity, and neurodegenerative conditions. It has also been employed in studies related to hypertension and cardiac function due to the involvement of NEDD4L in sodium channel regulation and blood pressure control.

Its specificity makes Nexinhib20 a valuable tool compound for validating NEDD4-family proteins as therapeutic targets and for screening downstream effects in signaling networks.