LY-411575 is a potent γ-secretase inhibitor widely used in neuroscience and oncology research. It effectively blocks the γ-secretase enzyme complex, which plays a key role in the cleavage of amyloid precursor protein (APP), leading to reduced production of amyloid-beta peptides. This makes it a valuable tool in studying Alzheimer’s disease pathways and evaluating potential therapeutic strategies targeting amyloid plaque formation. In cancer research, LY-411575 has shown activity against tumors dependent on Notch signaling. By inhibiting γ-secretase, it prevents the activation of Notch receptors, which are involved in cell differentiation, proliferation, and survival. This inhibition has demonstrated anti-tumor effects in models of T-cell acute lymphoblastic leukemia and other Notch-driven cancers, supporting its use as a research compound for probing oncogenic signaling pathways. Additionally, LY-411575 is used in stem cell and developmental biology studies to modulate cell fate decisions, as Notch signaling is critical in these processes. Its high potency allows for effective pathway suppression at low concentrations, making it suitable for in vitro and in vivo experimental models.