Dynamin IN-1 is primarily used as a cell-permeable inhibitor of dynamin GTPase activity. It is widely applied in cellular and molecular biology research to study endocytosis, particularly clathrin-mediated endocytosis, by blocking the scission of vesicles from the plasma membrane. This inhibition allows researchers to investigate the role of dynamin in intracellular trafficking, receptor internalization, and synaptic transmission. Dynamin IN-1 has also been utilized in neuroscience research to examine synaptic vesicle recycling and neuronal communication. Due to its ability to rapidly and reversibly inhibit dynamin, it serves as a valuable tool for acute experimental manipulation of membrane dynamics in live cells.