Used in research settings as a selective inhibitor of non-muscle myosin II, CL-82198 helps investigate cell motility, cytokinesis, and intracellular transport processes. It is particularly valuable in studies focusing on cancer cell migration and metastasis due to its ability to modulate myosin II activity without affecting other ATP-dependent motors. Its application extends to understanding cytoskeletal dynamics in both normal and pathological cellular functions. Due to its specificity, it serves as a tool compound in validating myosin II as a therapeutic target in various disease models.