CK-636 is a selective inhibitor of the Arp2/3 complex, a key regulator of actin polymerization in cells. Its primary application lies in cell biology research, where it is used to study actin dynamics and cytoskeletal reorganization. By inhibiting Arp2/3, CK-636 effectively blocks the formation of branched actin networks, allowing researchers to dissect the role of actin in processes such as cell motility, endocytosis, and intracellular pathogen movement. It has been widely used in studies involving cancer cell invasion and metastasis, as actin remodeling is crucial for tumor cell migration. Researchers apply CK-636 in vitro to examine how disruption of actin nucleation affects cell morphology and movement. Additionally, it serves as a tool compound in investigating immune cell functions, such as phagocytosis and T-cell activation, which rely heavily on cytoskeletal dynamics. Due to its specificity, CK-636 is also employed in high-throughput screening and mechanistic studies aimed at developing new therapeutics targeting actin-related diseases. However, it remains primarily a research chemical with no current clinical applications.