Used in research to study cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel function. It selectively blocks CFTR-mediated chloride transport, allowing scientists to investigate ion transport mechanisms in epithelial cells. Commonly applied in studies of fluid secretion, airway physiology, and intestinal ion transport. Also utilized to validate the role of CFTR in disease models and to assess potential therapeutic targets by inhibiting channel activity in vitro and in animal models.