CGK733 is primarily used in scientific research as a putative inhibitor of SIRT1, a member of the sirtuin family of proteins involved in cellular regulation, aging, and stress response. It has been investigated for its potential to modulate cellular senescence and enhance the cytotoxic effects of certain anticancer agents in experimental models. Researchers apply CGK733 in studies exploring pathways related to DNA repair, oxidative stress, and apoptosis, particularly in cancer cell lines. Due to its ability to sensitize cells to DNA-damaging agents, it is sometimes used as a tool compound to probe mechanisms of chemoresistance. However, its specificity and mechanism of action remain under investigation, and it is not used in clinical or industrial applications outside of laboratory research.