Used primarily as a selective inhibitor of myosin II ATPase, para-amino-Blebbistatin enables precise control over myosin-driven cellular processes in research settings. Its main application lies in cell biology studies involving cell motility, cytokinesis, and muscle contraction, where it helps dissect the role of myosin II without significant off-target effects. Due to improved photostability and reduced cellular toxicity compared to its parent compound, it is especially valuable in live-cell imaging experiments, allowing longer observation periods under microscopy. It is also employed in developmental biology to manipulate contractile processes in embryos and tissue models, supporting investigations into morphogenesis and tissue mechanics.