Used primarily as a selective inhibitor of myosin II ATPase, this compound is widely applied in cell biology research to study muscle contraction and cell motility. It helps disrupt actin-myosin interactions, making it valuable for experiments involving cytokinesis, cell migration, and intracellular transport. Due to its ability to reversibly inhibit non-muscle myosin II, it is also used in studies of tissue development, cancer cell invasion, and neuronal growth cone dynamics. Its specificity allows researchers to probe myosin-dependent processes with minimal off-target effects, especially in live-cell imaging and functional assays.