AM114 is primarily used in research settings as a selective inhibitor of the p97/VCP (valosin-containing protein) ATPase. This makes it a valuable tool in studying protein homeostasis, endoplasmic reticulum-associated degradation (ERAD), and cellular stress responses. Due to its ability to disrupt p97-dependent pathways, AM114 has shown potential in preclinical studies for targeting cancer cells, particularly those reliant on efficient protein degradation machinery for survival. It is often employed in cell-based assays to induce proteotoxic stress and evaluate the viability of tumor cells under such conditions. Its application extends to the investigation of neurodegenerative diseases where protein aggregation is a key feature, helping researchers understand the role of p97 in disease progression. AM114 is not used clinically but remains an important compound in mechanistic studies and drug discovery efforts targeting protein quality control pathways.