Used primarily as a highly effective irreversible inhibitor in biochemical research, particularly targeting serine hydrolases and other enzyme classes containing active-site serine residues. Its sulfonyl fluoride group reacts selectively with nucleophilic amino acids, making it valuable in activity-based protein profiling (ABPP) to identify and characterize functional enzymes in complex biological samples. Due to its small size and ability to penetrate cells, it is employed in live-cell labeling studies to monitor enzyme activity in native environments. Also utilized in chemical proteomics for target identification and validation in drug discovery. Its stability in aqueous solutions and selectivity under physiological conditions enhance its utility in both in vitro and in vivo applications.