Used in biochemical research to study mitochondrial function, particularly as an inhibitor of the adenine nucleotide translocator (ANT) in the inner mitochondrial membrane. It helps investigate mechanisms of oxidative phosphorylation and mitochondrial permeability transition pore (mPTP) formation. Due to its ability to induce mitochondrial dysfunction, it is also employed in studies related to cell death pathways, including apoptosis and necrosis. Its high specificity for ANT makes it a valuable tool in understanding energy metabolism and mitochondrial involvement in diseases such as ischemia-reperfusion injury and neurodegenerative disorders.