ENOblock is primarily utilized in research settings to study metabolic pathways, particularly those involving enolase, an enzyme crucial for glycolysis. It is used to inhibit enolase activity, allowing scientists to investigate the role of glycolysis in various cellular processes, including cancer cell metabolism. By blocking enolase, researchers can explore how cancer cells adapt to metabolic stress and identify potential therapeutic targets. Additionally, ENOblock is employed in studies focused on understanding the metabolic reprogramming that occurs in diseases such as diabetes and neurodegenerative disorders. Its application extends to drug discovery, where it serves as a tool to screen and develop compounds targeting metabolic enzymes.