BCAT-IN-2 is primarily used in research settings to study the role of branched-chain amino acid transaminase (BCAT) enzymes in cellular processes. It serves as a potent inhibitor of BCAT, making it a valuable tool for investigating metabolic pathways, particularly those involving branched-chain amino acids like leucine, isoleucine, and valine. Researchers utilize this compound to explore its potential in modulating amino acid metabolism, which is relevant in conditions such as cancer, neurodegenerative diseases, and metabolic disorders. Additionally, BCAT-IN-2 aids in understanding the biochemical mechanisms underlying BCAT activity, contributing to the development of therapeutic strategies targeting these pathways. Its application is largely confined to preclinical studies, where it helps elucidate the biological significance of BCAT in health and disease.