PDK4-IN-1 hydrochloride is primarily used in research settings to study the role of pyruvate dehydrogenase kinase 4 (PDK4) in cellular metabolism. It is a potent inhibitor of PDK4, an enzyme that plays a critical role in regulating glucose and fatty acid metabolism. By inhibiting PDK4, this compound helps researchers investigate metabolic disorders such as diabetes, obesity, and cancer. It is particularly useful in understanding how metabolic reprogramming in cancer cells can be targeted for therapeutic purposes. Additionally, it aids in exploring the potential of PDK4 inhibition in improving insulin sensitivity and managing metabolic syndrome. Its application extends to preclinical studies aimed at developing novel treatments for metabolic and oncological diseases.