NCT-503 is primarily utilized in biochemical research as a selective inhibitor of phosphoglycerate dehydrogenase (PHGDH), an enzyme involved in the serine biosynthesis pathway. Its application is significant in studying cancer metabolism, as many cancer cells upregulate PHGDH to support their rapid proliferation and survival. By inhibiting PHGDH, NCT-503 helps researchers investigate the role of serine metabolism in tumor growth and progression. It is also used to explore potential therapeutic strategies targeting metabolic dependencies in cancer cells. Additionally, NCT-503 aids in understanding the broader implications of serine biosynthesis in cellular processes and its impact on diseases beyond oncology.