Luciferase-IN-1 is primarily utilized in research settings to study and inhibit luciferase enzyme activity. It is a valuable tool in molecular biology, particularly in bioluminescence assays, where it helps in understanding enzyme mechanisms and pathways. Researchers use it to develop and optimize assays for drug discovery, enabling the screening of compounds that may interact with luciferase. Additionally, it aids in studying cellular processes and gene expression by modulating bioluminescent signals. Its application extends to cancer research, where it is used to investigate tumor growth and response to therapeutic agents in luciferase-expressing cell lines.