Leu-AMS is widely used in biochemical research as a potent inhibitor of leucyl-tRNA synthetase, an enzyme crucial for protein synthesis. It is particularly valuable in studies aimed at understanding the mechanisms of aminoacyl-tRNA synthetases and their role in cellular processes. Researchers utilize Leu-AMS to investigate protein translation and to develop potential therapeutic agents targeting bacterial or parasitic infections, as these organisms rely heavily on this enzyme for survival. Additionally, it serves as a tool in structural biology to explore enzyme-inhibitor interactions, aiding in the design of novel drugs. Its application extends to the study of antibiotic resistance, providing insights into overcoming challenges in treating infectious diseases.