HO-1-IN-1 hydrochloride is primarily used in research settings as a potent and selective inhibitor of heme oxygenase-1 (HO-1). This enzyme plays a crucial role in the breakdown of heme into biliverdin, carbon monoxide, and free iron, which are involved in various cellular processes. By inhibiting HO-1, this compound helps scientists study the enzyme's role in oxidative stress, inflammation, and cellular protection mechanisms. It is particularly valuable in investigating diseases where HO-1 activity is implicated, such as cancer, neurodegenerative disorders, and cardiovascular conditions. Additionally, it aids in exploring potential therapeutic strategies targeting HO-1 pathways. Its application is limited to laboratory studies and is not used in clinical or industrial settings.