HO-1-IN-1 is primarily used in research settings to study the role of heme oxygenase-1 (HO-1) in various biological processes. It acts as an inhibitor of HO-1, allowing scientists to investigate the enzyme's function in oxidative stress, inflammation, and cellular protection. This compound is particularly valuable in understanding the mechanisms underlying diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, where HO-1 activity is implicated. By blocking HO-1, researchers can explore potential therapeutic strategies and evaluate the enzyme's impact on disease progression. Additionally, HO-1-IN-1 is utilized in pharmacological studies to assess the efficacy and specificity of HO-1 inhibitors in preclinical models.