Used primarily in biochemical research, this compound is applied to study the inhibition of human dihydroorotate dehydrogenase (hDHODH), an enzyme critical in the de novo pyrimidine biosynthesis pathway. By targeting hDHODH, it helps researchers explore potential therapeutic strategies for diseases like autoimmune disorders, cancer, and viral infections, where rapid cell proliferation is a factor. Its application extends to drug discovery, where it serves as a tool compound to validate hDHODH as a target and to screen for novel inhibitors with improved efficacy and selectivity. Additionally, it aids in understanding the enzyme's role in cellular metabolism and its interaction with other biological pathways.