DO34 analog is primarily utilized in research settings to study its effects on specific biological pathways, particularly as an inhibitor of the enzyme DHODH (Dihydroorotate Dehydrogenase), which plays a key role in pyrimidine synthesis essential for cell growth and division. It is often employed in biochemical assays to investigate its potential in inhibiting enzymes involved in inflammation and cancer processes. Researchers use this compound to explore its impact on cellular processes, such as proliferation, apoptosis, or inflammation, providing insights into its therapeutic potential as an anti-cancer and anti-inflammatory agent. Additionally, it serves as a valuable tool in drug discovery, helping to identify and optimize lead compounds for further development with improved efficacy and specificity. Its application extends to understanding disease mechanisms and evaluating its efficacy in preclinical models.