DHODH-IN-3 is primarily used in research and development as a potent inhibitor of dihydroorotate dehydrogenase (DHODH), an enzyme crucial for pyrimidine biosynthesis. Its application is significant in studying cellular processes related to nucleotide metabolism, particularly in contexts like cancer research, where targeting DHODH can disrupt the proliferation of rapidly dividing cells. Additionally, it serves as a valuable tool in immunology and virology studies, as inhibiting DHODH can modulate immune responses and suppress viral replication. This compound is also explored in the development of therapeutic agents for autoimmune diseases and certain cancers, leveraging its ability to selectively inhibit metabolic pathways essential for disease progression.