Used in research settings as a selective inhibitor of protein arginine methyltransferase 5 (PRMT5), TH5487 is valuable for studying epigenetic regulation and RNA processing. It demonstrates potent cellular activity, making it a tool compound for probing PRMT5-dependent pathways in cancer models, particularly in hematological malignancies and solid tumors. Its ability to cross the blood-brain barrier also supports its use in neuroscience-related studies involving epigenetic mechanisms. TH5487 is primarily employed in preclinical research to assess target validation and therapeutic potential in oncology and other disease areas linked to aberrant methylation.