Used in biochemical research as a folate analog for studying folate-dependent enzymes and cellular uptake mechanisms. Serves as an intermediate in the development of targeted cancer therapies, particularly in antifolate drug design that inhibits dihydrofolate reductase (DHFR). Its alkyne functionality allows for click chemistry applications, enabling conjugation with fluorescent tags or other biomolecules for imaging and tracking in cellular systems. Also employed in the synthesis of antibody-drug conjugates (ADCs) for selective delivery to tumor cells overexpressing folate receptors.