Used in targeted cancer therapies, particularly in the treatment of solid tumors. It functions as a folate analog that inhibits dihydrofolate reductase, disrupting DNA synthesis and cell division in rapidly growing cancer cells. Its structure allows for improved cellular uptake through the folate receptor pathway, enhancing selectivity for tumor cells over normal cells. This compound is also investigated in combination with other chemotherapeutic agents to overcome drug resistance and improve treatment outcomes. Research explores its potential in receptor-mediated drug delivery systems due to its high affinity for folate receptors overexpressed in many cancers.