OSMI-1 is primarily used as a selective inhibitor of SUMOylation, a post-translational modification involved in regulating various cellular processes such as DNA repair, transcription, and protein stability. Due to its ability to block the SUMO-activating enzyme (E1), OSMI-1 serves as a valuable tool in studying the role of SUMO pathways in cancer and other diseases. It has shown potential in preclinical research for sensitizing tumor cells to chemotherapy and radiation by impairing DNA damage response mechanisms. Additionally, OSMI-1 is utilized in cellular and molecular biology experiments to probe SUMO-dependent signaling pathways and assess their impact on cell cycle regulation and apoptosis. Its application remains mainly in research settings, particularly in oncology and cell biology.