Used in research as a potent inhibitor of dihydrofolate reductase (DHFR), this compound shows promise in anticancer and antimicrobial drug development due to its ability to interfere with folate metabolism. Its structural features allow selective targeting of enzyme variants found in tumor cells and certain pathogens. It is also employed as a chemical probe to study cellular pathways involving nucleotide synthesis and cell proliferation. Ongoing studies explore its potential in targeted therapies, particularly for cancers resistant to conventional DHFR inhibitors.