RNF5 inhibitor inh-02 is primarily utilized in research focused on understanding and modulating protein degradation pathways, particularly those involving the RNF5 E3 ubiquitin ligase. This compound is employed in studies investigating cellular stress responses, protein quality control mechanisms, and the role of ubiquitination in diseases such as cancer, neurodegenerative disorders, and immune-related conditions. By inhibiting RNF5, researchers can explore its impact on the stabilization of misfolded proteins, endoplasmic reticulum (ER) stress, and the regulation of key signaling pathways. Additionally, it serves as a tool to evaluate potential therapeutic strategies targeting ubiquitin-proteasome system dysregulation. Its application is largely confined to preclinical and laboratory settings, aiding in the development of novel treatments for conditions linked to defective protein homeostasis.