Used in research and diagnostic laboratories to measure calpain enzyme activity in sheep tissue samples, particularly in muscle and blood. Helps in studying muscle degradation processes, such as those occurring during meat aging and post-mortem tenderization. Supports investigations into stress-related cellular changes and apoptosis in sheep models. Also applied in veterinary studies to assess muscle health and disease progression, including conditions linked to abnormal proteolysis. Enables quantification of calpain activity without the need for radioactive substrates, offering a safer and more convenient assay method.