Used for measuring 5-alpha reductase (5AR) enzyme activity in rat tissue samples, particularly in studies related to steroid metabolism. Commonly applied in endocrinology and pharmacology research to evaluate hormonal disorders, prostate health, and the impact of drugs on androgen regulation. Enables quantitative analysis of enzyme function in liver, prostate, and other tissues where 5AR is active. Supports investigation into conditions like benign prostatic hyperplasia and androgen-dependent diseases in preclinical animal models.