Used to measure 5-nucleotidase (5-NT) enzyme activity in rat biological samples such as serum, plasma, or tissue homogenates. This assay helps in assessing liver function and diagnosing liver-related disorders, particularly cholestatic conditions. It is commonly applied in preclinical research, toxicology studies, and drug development to monitor hepatobiliary health in rat models. The kit enables quantitative analysis, providing reliable and sensitive detection of 5-NT levels, which supports evaluation of enzyme kinetics and metabolic pathways in experimental settings.