Used for quantitative measurement of Hexokinase (HK) enzyme activity in rat tissue samples, particularly in liver, muscle, and blood cells. Widely applied in metabolic research to study glucose metabolism, insulin resistance, and diabetes-related pathways in rat models. Helps assess mitochondrial function and glycolytic flux in preclinical studies. Commonly used in pharmaceutical development and toxicology to evaluate drug effects on energy metabolism. Provides high sensitivity and specificity for detecting changes in HK levels under experimental conditions.