Used in biomedical research to measure malate dehydrogenase (MDH) enzyme activity in rat tissue samples, particularly from heart, liver, or skeletal muscle. Helps assess mitochondrial function and cellular metabolism in experimental models. Commonly applied in studies related to oxidative stress, metabolic disorders, ischemia-reperfusion injury, and drug toxicity. Provides a quantitative and sensitive method to monitor changes in MDH levels, supporting evaluation of organ damage or effectiveness of therapeutic interventions in rats.