Used in biochemical and medical research to measure glucose-6-phosphatase (G-6-P) enzyme activity in rat tissue samples, particularly in liver and kidney studies. Helps assess metabolic disorders related to glucose homeostasis, such as diabetes and glycogen storage diseases. Widely applied in preclinical drug testing to evaluate the impact of pharmaceutical compounds on glucose metabolism in rat models. Enables high-throughput screening with high sensitivity and specificity, allowing researchers to quantify enzyme activity changes under experimental conditions.