Used to measure 4-coumarate:CoA ligase (4CL) enzyme activity in plant samples through an enzyme-linked immunosorbent assay. This kit enables researchers to quantify 4CL levels in plant tissues, which is critical for studying phenylpropanoid metabolism. It supports research in plant biochemistry, especially in understanding lignin biosynthesis, flavonoid production, and plant defense mechanisms. Commonly applied in studies involving genetically modified plants, stress responses, and developmental biology where changes in metabolic pathways are analyzed. The assay provides a sensitive and specific method for high-throughput screening in agricultural and plant biotechnology research.